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Virginia Administrative Code
Title 9. Environment
Agency 20. Virginia Waste Management Board
Chapter 120. Regulated Medical Waste Management Regulations
10/25/2020

9VAC20-120-920. Representative of Biological Indicators.

A. One or more representative microorganisms from each microbial group shall be used in treatment efficacy evaluation.

1. Vegetative Bacteria.

- Staphylococcus aureus (ATCC 6538)

- Pseudomonas aeruginosa (ATCC 15442)

2. Fungi.

- Candida albicans (ATCC 18804)

- Penicillium chrysogenum (ATCC 24791)

- Aspergillus niger

3. Viruses.

- Polio 2 or Polio 3

- MS-2 Bacteriophage (ATCC 15597-B1)

4. Parasites.

- Cryptosporidium spp. oocysts

- Giardia spp. cysts

5. Mycobacteria.

- Mycobacterium terrae

- Mycobacterium phlei

- Mycobacterium bovis (BCG) (ATCC 35743)

B. Spores from one of the following bacterial species shall be used for efficacy evaluation of chemical, thermal, and irradiation treatment systems.

1. B. stearothermophilus (ATCC 7953)

2. B. subtilis (ATCC 19659)

Statutory Authority

Chapter 14 (§ 10.1-1400 et seq.) of Title 10.1 of the Code of Virginia.

Historical Notes

Derived from VR672-40-01:1 § 11.9, eff. June 29, 1994.

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